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After washes in PBS, the material was dehydrated in acetone and embedded in Epon.
After rinsing in 0.1 M phosphate buffer, the material was dehydrated in a graded alcohol series and acetone, and embedded in Epon 812 epoxy resin.
Finally, material was dehydrated through an ethanol series, and embedded in Spurr.
The fixed material was dehydrated in a graded alcohol series and embedded in Epon 812.
All material was dehydrated with ethanol and embedded in Epon 812.
This material was dehydrated in a graded series of ethanol (20% to 100%).
Similar(44)
The materials were dehydrated in an acetone series and embedded in the Spurr's resin (Electron Microscope Sciences, Washington, PA).
After staining with 1% uranyl acetate for 1 hour, the materials were dehydrated in an ethanol series and embedded in LR White resin (medium grade).
Fixed materials were dehydrated through a 20% increment of ethanol concentration every 15 min from 30%to90%0%, followed by two subsequent dehydration steps of 15 min each in absolute ethanol.
A final 60 μl of the material eluted was dehydrated in an RVC 2-25 CD rotational vacuum concentrator (Martin Christ GmbH, Osterode am Harz, Germany).
I was dehydrated.
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