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The pelleted material was boiled in sample buffer and separated by SDS-PAGE.
Then, the particulate material was boiled in 125 mM Tris pH 6.9, containing 6 M urea, 20 mM β-mercaptoethanol and 1% Tween 20 for 10 min. The samples were incubated at 4°C overnight to optimize the solubilization of the material.
200 mg plant material was boiled in 10 mL methanol and filtered.
Approximately 2 g of powdered material was boiled in 20 ml of distilled water in a water bath and filtered.
Sixty grams (60 g) of ground plant material was boiled gently with 1.6 L distilled water for approximately 3 h to reduce the volume to 200 mL.
200 mg of plant material was boiled in 10 mL distilled water and few drops of FeCl3 were added to the filtrate; a blue-black precipitate indicated the presence of Tannins.
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The plant material is boiled in water, and the volatile oils are carried over by the steam, condensed, and separated from the water.
Preparation of the aqueous extract of OH was as follows: 5 g of the powdered plant material were boiled with 100 mL of distilled water for 30 min.
Food materials were boiled rice and apple.
The silverware was boiled.
The composition of CBW depends on the type of cork material to be boiled and the number of boiling cycles, which differs from company to company.
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CEO of Professional Science Editing for Scientists @ prosciediting.com