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From all these zygotes, the genetic material was amplified and half of the amplified material (DNA) was sent to the other pilot study site for further processing (labelling and hybridization) and blind evaluation of its aneuploidy status under the code generated by the centre.
After cDNA isolation and purification using Qiagen PCR purification kit, input material was amplified using the MessageAmp II aRNA Amplification kit.
The sample material was amplified using Illumina primers to generate sufficient quantities required for hybridization.
For real-time PCR (qPCR), reverse-transcribed material was amplified in 25 µl Power SYBR Green PCR Master Mix Applied Biosystemm) using the 7000 Sequence Detection System (Applied Biosystem) or in 20 µl KAPA SYBR Fast qPCR Master Mix KAPA Biosystemss) using the RotorGene (Qiagen).
IBDV genetic material was amplified directly from bursa or cell passaged material.
The material was amplified and sequenced on an Illumina HiSeq 2000.
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Surface waves propagating from right to left (rigid soft material) were amplified at the boundary.
The cytb fragments from museum material were amplified using primer pair 2877, 2879.
The immunoprecipitated and input material were amplified using WGA2 and WGA1 GenomePlex Whole Genome Amplification Kits (Sigma).
Undergoing multiple rounds of copying should allow viruses to produce progeny more efficiently because the genetic material is amplified geometrically.
With total RNA as starting material, mRNA was amplified by T7-based in vitro transcription following the manual of TargetAmp™2-Round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI).
More suggestions(15)
material was fortified
material was complicated
material was produced
material was distilled
material was withdrawn
material were amplified
material was kept
material was subjected
material was discarded
material was found
material was verified
material was transferred
material was divided
material was selected
material was denoted
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