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This material was acidified by addition of 20 mL of 1% (vol/vol) sodium hydroxide acetic acid, hand-shaken for 2 minutes and centrifuged at 2000 rpm for 15 minutes.
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Proteolysis was acidified with 5% formic acid.
After incubation, the sample was acidified to pH = 7.0 with 6 N HCl.
After labelling, each solution was acidified by the addition of 3 µl trifluoroacetic acid and combined.
After cooling, the reaction mixture was acidified with HCl.
The mixture was acidified by diluted HCl solution and filtrated.
The filtrate was acidified by the addition of hydrochloric acid.
After washing it was acidified with conc.
After cooling, the mixture was acidified with 3 m aq.
The pH was acidified to 5 using 0.1 N HCl.
The resulting suspension was acidified with 2 m HCl.
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