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Electron microscopy and thermogravimetric analysis clearly revealed that the nitric acid-treated material under reflux conditions suffered the highest degree of degradation, such as, nanotube shortening and additional defect generation in the graphitic network.
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At last, the template was removed from the as-synthesized material by washing with ethanol under reflux for 24 h.
The grafting method consists in reacting a suitable organosilane with surface silanol groups belonging to a previously synthesized mesoporous material using an appropriate solvent under reflux conditions (Lim and Stein 1999).
The air-dried, powdered plant materials (20 kg) were extracted with 95% EtOH under reflux three times.
Extractions under reflux were repeated three times on fresh portions of the material.
The dried plant materials (25 g) were extracted with distilled water (220 mL) two times under reflux for 1 hr and were then filtered through filter paper.
SBA-15 was prepared under reflux, resulting, unlike SBA-15 prepared under autogenous pressure, in a mesoporous material with thicker pore walls but with a slightly smaller unit cell parameter and pore diameter.
Here the reactant and catalyst were put in ethanol under reflux for suitable time (cf. Tables 2 and 3) until the starting materials were no longer detectable by TLC.
Plant material (50 g) was placed in a 250-ml round bottom flask with 100 ml of MilliQ® water and boiled under reflux for 45 min.
Reactions were carried out in batch mode under reflux.
The reaction mixture was heated under reflux for 1 h.
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