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We recently reported the presence of M6G in the matrix of bovine adrenal chromaffin secretory granules, as well as in the material secreted from chromaffin cells [15].
In addition to examining cement protein gene expression levels, material secreted from barnacles at the substrate interface was analyzed using mass spectrometry.
In addition to the epidermal staining, PNA stained material secreted from the cement gland, which is also positive for a mucin, muc5e (supplementary material Fig. S3).
Mucins can be isolated based on their density, so to further enrich the PNA-positive material secreted from the epidermis we separated the samples using caesium chloride (CsCl) density gradient centrifugation.
In our attempt to characterise the nature of PAS-positive material secreted from the UVD, we excluded glycogen by showing that PAS staining persists after treatment with the glycogen-digesting enzyme, diastase.
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Since there was no difference in the plating efficiency when transformant and parental cell lines were tested in their conditioned or fresh unconditioned media, the increase in adhesiveness of the transfectants arises almost exclusively from the cells themselves and not from material secreted into the conditioned medium.
Secretory cells grown in culture with the protocol described in this study, identified cells with spontaneous degranulation, where the material secreted was observed emerging from the cell surface, in fact, rapidly expanded (5 to 20 s), forming spherical structures (arrows in Figure 1(c)) which reached diameter similar to those observed previously in other biological models [ 46, 47].
The mucoid layer is deposited during passage through the oviduct whereas shell coat material is secreted from endometrial glands within the uterus and the uterotubal junction.
Firstly, IS phospholipids may represent pools of surfactant with different temporal origins, with some material newly secreted from the alveolus combined with previously secreted surfactant embedded within the mucus layer.
Arthropod exoskeletons are made of cuticle, a non-cellular material secreted by the epidermis.
It has been proved that H2C2O4 is the primary pathogenic material secreted by S. sclerotiorum [ 22- 24].
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Justyna Jupowicz-Kozak
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