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The Eameses' commitment to fully knowing the material resolved early technical issues with molded plywood, enabling the eventual mass production of inexpensive furniture.
Lysates were spun at 14,000 rpm for 10 minutes to remove insoluble material, resolved by 7.5% SDS-PAGE, and probed with PARP antibodies.
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President Bush's choice to head the Securities and Exchange Commission helped an online distributor of sex-related material resolve troubles with the Nasdaq stock market, commission records show.
Nanoparticle transfer of genetic material resolves these issues, and the range of nanoparticles available may allow this method to be optimized to a higher efficiency than other gene delivery methods.
As a second purification step, this 32 kDa material was resolved by reducing PAGE, after which the material migrating at approximately 18 kDa was recovered.
After 60 days of the composting process, the material was resolved and the average temperature of the pile was 50 °C.
The forces applied to the material are resolved into forces along and parallel to the tow directions.
After three washes of the beads in lysis buffer the immunoprecipitated material was resolved by 12% SDS-PAGE and visualized by autoradiography.
Beads were washed in RIPA buffer and the IP material was resolved by 14% SDS-PAGE, transferred onto PVDF membranes, and visualized and quantified using a PhosphorImager.
Either 20 or 80% of the precipitated material was resolved by SDS-PAGE analyzedyzed by Western blotting with an anti-CD4 (H370, Santa-Cruz, CA) and polyclonal antibodies generated against the C-terminal domain of PLSCR1, respectively.
Protein lysates from Leishmania transfectants expressing either wild type or mutant forms of Ufm1 (Ufm1ΔC8A, Ufm1Δcontaininging HA-epitope tag were incubated with anti-HA affinity gel and the immunoprecipitated material was resolved on SDS-PAGE and the immunoblots were probed with anti-LdUba5 antibodies.
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