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The promise of this technology has been hampered by the large amount of biological material required for the experiments (more than 50 μg of total RNA per array).
Given the significant amount of sorted material required for the experiments and the absence of robust differences between mixed cells and PS7, we have chosen to save a more thorough investigation of these data for another manuscript.
The materials required for the Consortium application are almost identical to those materials we require.
Additional file 1: Reagents and materials required for the test.
We conclude therefore that the One-Direct system can further push the lower limits of material required for probe synthesis for microarray experiments to 50 pg of input when using high quality RNA, without significant impact on resulting information generated.
This modification was necessary because of the large quantities of material required for PMMA bone cement biofilm experiments.
This would economise the material required for serviceability design.
The hybrid methodology was combined with a microscale experimental platform, to significantly reduce both the number of experiments required as well as the time and material required for full kinetic parameter estimation.
This happens because the materials required for high temperatures and pressures typically metals conduct heat.
Become familiar with the few materials required for art therapy.
required for the job.
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CEO of Professional Science Editing for Scientists @ prosciediting.com