The same applies to material pooled with those overseas secret services (beyond the old America/Canada/Australia loop) with whom the British agencies have operational links.
RNA isolated at each time point of the ES and GCNF−/− time series experiment from material pooled from two independent 10 cm dishes each with ∼75 million cells were used to probe Mouse Array Version 2. For each time point, two dishes were pooled to harvest 0.5 1.0 mg of RNA for the arrays.
Each sample consisted of material pooled from six plants.
Each sample comprises biological material pooled from 5 different plants.
Each of the duplicates contained material pooled from multiple mice.
In each case, RNA was extracted from material pooled from 4 separate plants.
All induction experiments were repeated three times, the material pooled and subjected to two separate RNA extractions.
There were four replicates of each biological sample, each containing material pooled from five to eight insects.
Input material pooled from five replicate cultures was subjected to immunoprecipitation with anti-FLAG or control IgG1 antibody (see above).
Samples were collected in three biological replicates per genotype and each sample (65 250 mg) consisted of material pooled from six plants.
