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RNA isolated at each time point of the ES and GCNF−/− time series experiment from material pooled from two independent 10 cm dishes each with ∼75 million cells were used to probe Mouse Array Version 2. For each time point, two dishes were pooled to harvest 0.5 1.0 mg of RNA for the arrays.
Each sample comprises biological material pooled from 5 different plants.
Each of the duplicates contained material pooled from multiple mice.
Each sample consisted of material pooled from six plants.
In each case, RNA was extracted from material pooled from 4 separate plants.
Input material pooled from five replicate cultures was subjected to immunoprecipitation with anti-FLAG or control IgG1 antibody (see above).
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Metagenomic analysis: study of a microbial community by sequencing the genetic material pool from an environmental or clinical sample.
Plant material was pooled from several plants.
Synovectomy material was pooled from two cases, from two MCP joints in one case and from two PIP joints in the other case.
Plant material was pooled from six different cuttings bases for each sample for isolation of total RNA using the TaKaRa RNAiso reagent [ 46].
Additionally, to minimize changes in transcript abundance due to diurnal and circadian rhythms, RNA was pooled from material harvested at six different circadian time points over a 24-hour period.
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