WHA whole animal, PYT phytoplankton, ZOO zooplankton, SED sediment, PLT plant material, PER periphyton, MAC macroinvertebrates, LUP lupin, RCP commercial redclaw pellet, SOY soybean, CHK chicken feed.
Sixty colonies from the SFS and NFS sun and shaded area plates (corresponding to 10−3 10−4 g soil or plant rhizosphere material per plate) were screened for: 1) ACC deaminase production, 2) biofilm formation, 3) phosphorus solubilization and 4) halophilic growth.
The content of endospore-forming bacteria was determined after heat treatment of the soil or plant material suspension at 80°C for 30 min. Tryptic Soy Agar (TSA) plates were inoculated with 100 µL of these suspensions, corresponding to 10−3 10−5 g soil or plant rhizosphere material per plate.
As described earlier, SFS 1 sun and shadow, SFS2 sun and shadow, NFS5 sun and shadow, and NFS7 sun and shadow samples were pooled and treated at 80°C for 30 min. TSA plates were inoculated with 100 µL of these suspensions, corresponding to 10−3 g soil or plant rhizosphere material per plate.
A total of 40 sample trees at each site (20 per plant material treatment) were selected for measurement.
Each extraction yield was calculated and expressed in oil weight per plant material weight with Humidity Free Base (% p/p HFB) (Guimarães et al. [2008]).
Agroinfiltration was carried out in such a way as to include all possible variations in vIL-10 expression due to the state of the plant/leaf material (two plants, three leaves per plant, and randomized leaf areas including the tip, center and base).
Quantitative analysis of OPDA, JA and JA-Ile was done using 500 mg of homogenized plant material per sample as described [ 24].
JA, JA-Ile and OPDA were determined as described by [ 43] using 1 g fresh weight of homogenized plant material per sample.
