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No LOH could be detected, which is in agreement with the heterozygous nature of the mutation in tumour material observed by DNA sequencing.
As in the case of the TB4 domain C1564Y MFS-associated mutant, none of the TB5 domain MFS-associated GFP-Fbn constructs were detected in the microfibril network assembled by the FS2 fibroblasts in co-culture (Fig. 3, Panels K and L), consistent with the lack of secreted material observed by western blotting of medium samples.
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Moreover, the melt viscosity of the experimental material was observed by using an off-line rheometer.
Important structural properties and homogenity of each material were observed by electron microscopy and analyzed by image analysis.
Structural and functional changes of the material were observed by Raman spectroscopy and voltammetry.
Crystallization, size distribution and absorption photoluminescence (PL) of this material are observed by XRD, TEM, and spectrometer respectively.
Only minor degradation of the H-SAPO-34 material was observed by X-Ray diffraction or infrared spectroscopy after 5 sequential test-regeneration cycles at 400 °C and 550 °C, respectively.
A high hardness value on the nitrided surface and in the nitrided layer in comparison with a low value of hardness in the core material was observed by Vickers microhardness testing.
In long adventitious roots (105-130 mm in length), barrier to ROL was well formed within 24 hours under stagnant deoxygenated conditions and dark granules of high-density material were observed by transmission electron microscopy in the spaces between the exodermal cells and also between the sclerenchyma cells in roots at 48 hours after the stagnant treatment (Shiono et al. 2011).
The resulting mixture was stirred at 0 °C until disappearance of the starting material was observed by TLC (about 1.5 h).
The solution was stirred at room temperature until complete consumption of the starting material was observed by TLC.
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