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Four were HCs (Fig. 2A; supplementary material Movie 3), eight were iACs (Fig. 2B; supplementary material Movie 4) and four were DACs (Fig. 2C; supplementary material Movie 5).
Using this line, supplementary material Movie 2 illustrates how the ER maintains its integrity during cell division, in contrast to the nuclear envelope (supplementary material Movie 1).
The migratory features of Xenopus NCCs under 2D (Fig. 2a,b; supplementary material Movie 1) and 1D (Fig. 2c,d; supplementary material Movie 1) culture conditions were compared.
As paxillin intensity stabilized, Myosin 1E intensity went down (supplementary material Movie 6; Fig. 2F).
This result was confirmed by confocal microscopy (supplementary material Movie 3).
Fig. 7C shows a typical kymograph of the flow (see also supplementary material Movie 1).
Figure 4 and Supplementary Material, Movie S1 were created as follows.
We followed 50 iACs throughout the multipolar phase (Fig. 4A; supplementary material Movie 4).
Cell tracking also clearly demonstrates this (Fig. 4A-D′, supplementary material Movie 3).
Within each domain, cells traveled along similar trajectories (Fig. 6K, supplementary material Movie 8).
Our prediction was confirmed by long-period time-lapse studies (Fig. 6; supplementary material Movie 4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com