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For additional information see Supplemental Material, "Microarray study".
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Here, we discuss recent developments in materials microarray technology and their applications in stem cell engineering.
As an alternative, materials microarray technology has received great attention because it allows for high throughput materials synthesis and screening at a reasonable cost.
Slightly different results could be expected with different source materials, microarray platforms or hybridization conditions and optimization of certain parameters or larger number of replicates may be beneficial for particular systems.
Universal reference RNA has been previously established as a standard reference material for microarray experiments, proving an ability to effectively hybridize to a large fraction of microarray spots [ 30].
On occasion this may reflect the accuracy of the selection of material for microarray, resulting in false negatives.
JJ provided the plant material for microarray hybridizations.
LB carried out and supervised greenhouse experiments, material collection, microarray hybridization, and Cd analysis.
YG conceived of the study, participated in its design, carried out the material preparation, microarray data analysis and drafted the manuscript, participated in QRT-PCR.
Normal and tumor samples were selected if there was sufficient material to microarray profile both laser capture microdissected samples and bulk tissue samples.
This RNA amplification methodology was originally developed as a method to increase very small amounts RNA samples to produce enough material for microarray hybridization [ 53].
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