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Concentrations of aU in cores 17PC and 31BB are substantially greater than would be expected if the uranium present was derived solely from uranium incorporation into foraminiferal calcite or organic material (Methods) (Fig. 2).
Regarding this material, methods HI and SS showed depth of cure of 3 mm while PD and LI, 2 mm.
For further details on computational approaches see Supplementary Material (Methods M1 and M4).
The molecular structures of these compounds are included in supplementary material (Methods S1).
The apparatus and a sample run are depicted in supplementary material (Methods S1).
An example run is shown in supplemental material (Methods S1) wherein FCCP was added at the end demonstrating the expected transient increase in O2 consumption with chemical uncoupling.
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The reader will find the mathematical formulation of these statements at the beginning of the Material & Methods section.
As described in Material & Methods, gangliosides were extracted by several solvent partitions and their oligosaccharides were digested by an endoglycoceramidase.
The reproducibility of the assay is expressed in intra- and inter-assay percent coefficient of variation (CV) as explained in Material & Methods.
After an exhaustive quality control and normalization steps (see Material & Methods), Spotfire Decision Site software was used to identify differentially expressed genes.
In our study, it was shown (see Material & Methods and Table S5) that pantothenate and biotin were indeed required for CIRM-BIA1T growth, in agreement with genomic data.
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