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In order to trace the progeny of early AspM expressing cells, we generated a transgenic mouse line, in which the inducible Cre recombinase (CreERT2) [24], [25], [25] -i.e. transiently activated by injecting mice with Tamoxifen (Tam) [26], [27]-, was placed under the control of AspM cis-acting regions (Figure S1A and material method section for a detailed description of the construct).
Cut-off values are defined in material method section.
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The covariate definitions were standardised across studies (supplementary material; methods section).
This bifurcation can be of several types as discussed in the Material and Method section.
C2C12 were transfected with the siRNAs as indicated in the Material and Method section.
In "Materials and methods" section the materials and methods are explained.
More details are described in materials and method sections.
The reader will find the mathematical formulation of these statements at the beginning of the Material & Methods section.
The 3 different methods are described in detail in "material and methods" section.
Each of them implements two statistical controls and are detailed in the Material and Methods Section.
Monocytes were purified from PBMCs and irradiated as indicated in the Material and Methods section.
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