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Plant derived smoke was generated by burning of plant material (leaf, straws etc) in a specially designed furnace, and seeds were treated with this smoke for different time duration.
For visualization of lignin distribution, plant material (leaf blades, petioles, and stem) was free-hand sectioned with a razor blade.
The mean contribution of leaf material (leaf blade and sheath) to total dry biomass among the genotypes was 63·8%% (ranging from 42·2 to 80·4 %) at the AG stage, 55·6 % (36·3 78·8 %) for PB stage and 36·1%% (15·8 63·2 %) for SS (Table 1).
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Leaf material (one leaf per individual) was collected from a total of 290 individuals and stored in silica gel.
There is no shortage of raw material for leaf mould at Glebe Cottage: two sides of the garden are surrounded by trees.
When tightly folded young leaves are used as starting material, the leaf pieces are often completely covered with regenerating plant material (Fig. 1C and 1D).
Other scientists have looked at materials like leaf compost or manure, while Professor Brown favors composts high in iron content, like the sludge from wastewater treatment.
Purified chloroplasts were isolated from approximately 8.2 g of Nandina leaf material and 30.8 g of Platanus leaf material, following the sucrose gradient protocol of Jansen et al. [ 7].
These values were determined using silica dried leaf material analyzed together with the internal standard consisting of leaf material of a specimen that was reported to be tetraploid [ 30].
Indoors, the light-gathering material in the leaf cells turns to offer the maximum surface area to the limited light.
With FW the weight of fresh leaf material.
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