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AMD assisted with material isolation from drought stressed plants.
CT-U and KF assisted with material isolation from developmental series and environmental treatment experiments, respectively.
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The procedure of plant material, DNA isolation, sequencing and SNP identification were performed as described previously.
Table 4 Plant material and isolation Family Species Plant parts Place of origin Plant wt.
Table 6 Plant material and isolation Open image in new window.
For additional information, see Supplemental Material, "RNA isolation and QRT-PCR".
Due to limited amounts of obtained material, microvessel isolation could only be performed from CAD 2 5 and Ctrl 1 4 samples.
Randomly picked apt clones from selection plates were further propagated on 2-FA media to provide enough material for isolation of genomic DNA and sequencing analysis of APT locus.
In 2007, Miller et al. [ 31] decided to establish peripheral whole blood to be a suitable material for isolation of mRNA for genetic testing use in the diagnosis of certain diseases of the cardiovascular system, including LQTS.
Although primary cultures using species with larger brains (e.g., pigs, cows) generate more material per isolation procedure and can generate larger TEER and smaller permeability coefficients than primary murine models, many in vivo BBB drug transport studies are performed in murine models and a large selection of murine antibodies exist.
In order to find out the best material for isolation of the 9-LOX gene, N. benthamiana leaves were infiltrated with a series of mixtures of viral provectors (detailed description see Experimental procedures): 1) 3'-provector; 2) 3'-provector and integrase provector; 3) 3'-provector and 5'- provector; 4) 3'-provector, 5'-provector and integrase provector.
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