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In biomass refinery, lignocellulosic material is fractionated to obtain the main components (cellulose, hemicelluloses, and lignin).
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The gas and particulate phase material was fractionated by HPLC into nonpolar, moderately polar and highly polar chemical fractions.
The ammonium sulphate precipitated material was fractionated by ion-exchange (a); and the most active peaks were separated by gel filtration (b); the main active peak was further analyzed for purity by HPLC (c).
Cells were lysed and soluble material was fractionated by centrifugation through sucrose gradients.
The bound material was fractionated by SDS-PAGE and analyzed by IB with anti-puromycin antibody (top panel).
One fifth of the amplified product (corresponding to 2% of the original material) was fractionated on 6% polyacrylamide gels in Tris-borate-EDTA.
Samples were spun for 15 min at 20,000 x g to remove insoluble material, and the solubilized material was fractionated by gravity flow over a 1 ml Sephacryl-S-300 column pre-equilibrated in column buffer (50 mM HEPES [pH 7.5], 200 mM KOAc, 15 mM MgOAc2, 1 mM DTT, and 0.25% digitonin).
Until then, the road ahead is full of booby traps and hurdles in a society that is fractionated.
The cracked reactor effluent is fractionated in a distillation column.
The polymers were fractionated by fractional precipitation.
This amount of HA-FUSWT in the cytoplasmic FUS was similar to that seen when endogenous HEK293T cells were fractionated (Supplementary Material, Fig. S3).
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