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When used directly on material from positive blood cultures, the sensitivity for the Oxoid Dryspot Pneumo Test was 77% (17/22).
Volumes of material from positive blood culture bottles ranging from 25 to 300 μL were added to 0.5 mL plasma from horse, rabbit or human origin.
Our strategy for GPC in clusters was to immediately culture the blood bottle material from positive blood culture bottles onto solid blood agar plates and to initiate a TCT.
The first evaluation phase involved comparing latex agglutination tests directly on material from positive blood culture bottles, previously shown to be reliable when performed on grown colonies [ 13, 14].
For streptococci the strategy was to identify blood cultures containing beta-haemolytic streptococci, enterococci and pneumococci by using agglutination kits directly on material from positive blood culture bottles; with repeated testing on colonies after 6 hour incubation on solid blood agar.
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This same region was then amplified from fecal material from coronavirus-positive healthy cats.
Formalin-fixed, paraffin-embedded surgical biopsy material from tumour-positive cases was selected for immunohistochemical analysis.
S1 PEDV sequencing was completed on PCR-positive feed challenge material from both the Positive control group and the Treatment group, as well as an intestinal homogenate from the index piglet in the Treatment group.
In addition, different levels of Notch expression itself (see Fig. S3A in the supplementary material), probably resulting from positive feedback (Bishop et al., 1999), might contribute to the differential signaling levels.
For electron microscopy a piece of paraffin embedded material from two MCPyV positive primary MCC samples was used.
In each cell, the bit-storing capacitor is coated with a thin film of a material that polarises (changes from positive to negative charge or vice versa) when a voltage is applied to it.
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