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Exact(5)
We did not analyse material from metastatic sites.
(5, 12) Furthermore, the significance of performing genomic testing for somatic mutations in KRAS, BRAF and PIK3CA is recognized in molecular target therapy,(13)– (16) but material from metastatic tumors is not always included in the testing.
Not many patients will undergo procedures that allow the collection of material from metastatic sites, and no single centre is likely to be able to collect significant numbers of quality specimens for research.
There are many reasons for these gaps in our knowledge, including the following: A lack of suitable model systems reflecting the complexity and diversity of breast cancer; Limited access to clinical material from patients before and, in particular, during treatment; A severe lack of material from metastatic deposits made available for studies of target hits and biological response to therapies.
To generate primary Ewing sarcoma cell cultures, biopsy material from metastatic relapse tumours in two adolescent patients (MS-PES-1, MS-PES-3) was dissected into 1 2 mm fragments, incubated in trypsin (0.05%)/EDTA (0.02%) solution (PAA, Cölbe, Germany), and passed through a cell strainer.
Similar(53)
Routine sampling of metastatic tissue will give us the opportunity to establish bio-banks of biopsy material taken from metastatic sites.
We also speculate on the future translational implications for biopsy of suspected metastatic lesions in the context of clinical trials and the establishment of bio-banks of biopsy material taken from metastatic sites.
CRC specimens (Table 1) were derived from the following sources: Group A (n = 135) included material from patients with metastatic CRC that was prospectively investigated for KRAS codon 12 and 13 mutations to predict patient eligibility for treatment with anti-EGFR antibodies, according to current guidelines [5], [6].
In an attempt to investigate the association of PTZK/ZK therapeutic activity with tumour angiogenesis and anaerobic metabolic pathways, paraffin-embedded material from 164 patients with metastatic colorectal adenocarcinoma were recruited in the CONFIRM 1 and 2 trials.
Prospectively collected biological material from either primitive tumor or metastatic disease will be stored until the end of patients' accrual.
SCHACK--Ann, 80, died November 13, from metastatic lung cancer.
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