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Accordingly, the 42 kDa insoluble Wnt1 isoform was occasionally seen on Western blots of insoluble material from control gfp/Wnt1 transfected 293 cells (not shown).
Experiments were performed with isolated salivary gland material from control as well as from experimental animals.
(C ) Immunoblots of detergent soluble (left) and insoluble (right) material from control or Vps4A & Vps4B siRNA-treated HeLa cells.
For the quantification of proline, between 20 and 30 mg dry leaf material from control and salt-treated plants were used.
Cultures of Jurkat cells (2 × 10 cells ml−1) following no treatment, treatment with 400 μg ml−1 membrane vesicles, or treatment with analogous material from control serum were removed from the flask after 24 h.
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Aliquots of lysates from cells incubated in medium containing ∼38 μg/mL of BSA-SWNT material (and from control cells incubated in medium that did not contain BSA-SWNTs) were subjected to SDS-PAGE at 100 V for 2 h, and dark bands of material were evident only at the loading well/stacking gel interface of lanes loaded with lysate from SWNT-treated cells.
Likewise, normal white matter material from four control cases was collected.
This shortfall might be resolved by using biopsy material from the control population, but this raises both ethical difficulties and technical problems in relating the biopsy site to the mammographic feature.
It passed a resolution telling buyers of tin not to source their raw material from mines controlled by Congolese militia leaders.It was highly unusual for the council to issue an order to anybody except governments.
To study whether cfDNA levels were already decreased at the early stages of the disease, we determined cfDNA levels also in sera from eRA patients (n = 34) using the Alu115 and β-actin205 primer sets and performed additional analyses with material from healthy controls (n = 29).
It was performed on formalin-fixed, paraffin-embedded (FFPE) autopsy brain material from 30 controls without neurological disease or brain lesions and 33 MS cases (Table 1).
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