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We are grateful to K. Baker, L. Davalos, D. Hayman, E. Koilmani, Y. Liu, A. Peel, R. Ransome and A. Rodriguez for providing material for sequencing.
In other cases, researchers create genomic or complementary DNA (cDNA) libraries to identify interesting genes or to provide cloned material for sequencing.
Five obese, hyperleptinemic and five obese, nonhyperleptinemic mares were selected to study the possibility of polymorphism in exon 2. First, in experiment 2, forward and reverse primers were designed from the Bos taurus leptin gene (GenBank Accession # U50365) to identify and subsequently clone the equine leptin gene to provide material for sequencing.
CC collected and processed finch material for sequencing.
Once DNA is extracted, small yields require amplification to obtain enough material for sequencing.
This final point is particularly relevant to researchers who are considering the use of WGA amplified material for sequencing.
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BAC libraries from four rice varieties and 14 wild rice accessions were used as primary materials for sequencing the whole genomic regions of the Hd6 gene (Table 1).
While single cell transcriptome sequencing is becoming more common, sequencing of individual embryos is not typically performed due to a scarcity of biological material necessary for sequencing library preparation.
To expand the number of specimens available for the present study, prostatectomy samples that had been formalin fixed and paraffin embedded (FFPE) were used, with modifications made to standard protocols to increase the yield of material used for sequencing.
We first assess the phylogenetic relationships between the sequences of the viruses present in the plasma, the monocytes, and the CD4+ T-cells during cART (see Fig. S2 in the supplemental material for sequence alignments).
The V3 amino-acid sequences of the CXCR4-using viruses found in the CD14high CD16− monocytes revealed a QR insertion immediately before the GPGR crown in two patients, and a net charge of ≥+7 in three patients (see Fig. S2 in the supplemental material for sequence alignments).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com