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We first assess the phylogenetic relationships between the sequences of the viruses present in the plasma, the monocytes, and the CD4+ T-cells during cART (see Fig. S2 in the supplemental material for sequence alignments).
The V3 amino-acid sequences of the CXCR4-using viruses found in the CD14high CD16− monocytes revealed a QR insertion immediately before the GPGR crown in two patients, and a net charge of ≥+7 in three patients (see Fig. S2 in the supplemental material for sequence alignments).
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The study is still collecting data and biological material for sequencing and genotyping.
TTAS not only provides enough material for sequencing library construction, but faithfully retains the copy number profiles and terminal information of the DNA fragments.
In other cases, researchers create genomic or complementary DNA (cDNA) libraries to identify interesting genes or to provide cloned material for sequencing.
Five obese, hyperleptinemic and five obese, nonhyperleptinemic mares were selected to study the possibility of polymorphism in exon 2. First, in experiment 2, forward and reverse primers were designed from the Bos taurus leptin gene (GenBank Accession # U50365) to identify and subsequently clone the equine leptin gene to provide material for sequencing.
Our selection of species for this project was guided by phylogenetic position, availability of material for sequencing, and the suitability of the species for subsequent experimental analysis.
The advent of MDA technology for amplification of single cells makes it possible to acquire enough genomic material for sequencing of specific genes, in this case the SSU rRNA and the gyrB gene, and ultimately sequencing the entire genome.
CC collected and processed finch material for sequencing.
Due to the availability of the raw material for sequencing, the run was continued to completion.
The assemblies are named volc454, volcV and volcIDBA (see Supplementary Material for sequencing and assembly details).
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