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Archived serum appears to be a useful source of clinical material for retrospective analyses but may require controlling for the duration of sample storage.
However, formalin-fixed, paraffin-embedded (FFPE) tissue is available in a large quantity, making it the ideal material for retrospective studies.
DNA derived from FFPE tissue represents a highly valuable source of material for retrospective studies.
DNA derived from FFPE is an extremely valuable source of material for retrospective studies, but is often highly degraded.
The method can provide several advantages such as: 1) the use of archival material for retrospective studies on the epidemiology of PCV2 infection in the wild; 2) histological sampling to be also tested and preserved for DNA assessments (simplifying the work of both technicians and hunters, as one sample can cover two fields of investigation).
Similar(55)
For 15 patients diagnosed before 2004, material was available for retrospective sequencing analysis.
At diagnosis no metaphases were found after culture and no frozen material was made available for retrospective molecular cytogenetic techniques.
Finally, attention is drawn to some of the technical difficulties in detecting basement membrane antigens in formalin-fixed tissue, the material most frequently available for retrospective study.
Although the RNA extracted from FFPE blocks is often compromised, we demonstrated the robustness of miRNA profiles in FFPE material which could provide a source of study material for large scale or retrospective studies.
The approval of the ethical committee includes a waiver of consent for retrospective TMA based studies based on material archived at the Institute of Pathology, University of Bern, Switzerland.
Unfortunately, of 67 samples (out of 324 NWSs) too little material was left for RT-PCR after thawing, leaving 257 samples for retrospective analysis.
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