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Samples from wild type and mutant plants were pooled separately to obtain enough material for nuclei isolation for each replicate.
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The formation of the blastopore, stomodeum and proctodeum is described from sectioned material using light and transmission electron microscopy as well as whole-mount material stained for nuclei and gene expression.
Fortunately, a number of ingenious experimental methods have been developed to allow isotropically averaged (i.e., high-resolution) NMR spectra to be recorded for powder samples, in which a narrow line is observed for each structurally distinguishable environment in the material for the type of nucleus of interest.
This composite hydrogel can cross-link in situ and is therefore an ideal material for injection as a nucleus replacement.
For pDNA delivery, a nuclear localization signal (NLS) was added to the biopolymer structure to facilitate active translocation of the genetic material towards nucleus.
Chitosan hydrogels (CHs) have been considered as a potential implant material for replacement and repair of the Nucleus Pulposus (NP) within the intervertebral disk.
Non-viral gene carriers are generally believed to be highly dependent on cell division for delivery of genetic material to the nucleus [ 27– 29].
This may reflect a redistribution of material between the nucleus and the cytoplasm, and/or a reorganization of the nucleus.
The raw material for CERN's antimatter factories is made by shooting protons, or naked hydrogen nuclei, into a iridium target, but "instead of speeding them up," Dr. Landua said, "we slow them down".
When lemma was used as bombardment material for subcellular localization, some of the BeMADS proteins were localized to the nucleus.
Yamada et al. [11] and Yamada and Matsumura [12] modeled neo-Hookean material for a cell on a substrate being stretched with Pa, 5100 Pa, and 775 kPa for the cytoplasm, nucleus, and substrate, respectively.
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