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Clinical specimens frequently contain small amounts of RNA and amplification is required to obtain sufficient material for expression analysis.
Small surgical biopsies, fine needle aspirates, cyto-lavages, punch biopsies and blood samples often yield only 1 4 μg quantities of RNA as starting material for expression profiling.
Stumpy forms were purified from host blood by DE52 chromatography [ 39] at 37°C and then incubated for 1 h at 37°C in HMI-9 [ 40] to allow the cells to recover from the purification process, this providing the starting material for expression analyses during their differentiation to procyclic forms.
Basal-like breast cancer has been extensively characterized on the basis of gene expression profiles, but it is becoming increasingly common for these tumors to be defined on the basis of immunohistochemical (IHC) staining patterns, particularly in retrospective studies where material for expression profiling may not be available.
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The plant material used for expression profiling (bark tissue from tree leaders) was harvested in a randomized fashion to minimize bias due to the sampling procedure.
FACS sorting of 2 did not yield enough genetic material for gene expression and mutation analyses; thus gene expression studies were preferentially completed.
Preserving plant material for gene expression analyses requires that the tissue be prepared and stored in a manner that maintains the integrity of RNA.
Material for gene expression analysis was snap-frozen in liquid nitrogen within two minutes.
Diagnostic biopsies and material for gene expression were taken from adjacent locations with needle by Computer Tomography and/or ultrasound guidance.
We used the total RNA from about 1500 hybrid cells (12 colonies) and equal numbers of hFL and HEF cells (donors 19 and 24) as starting material for the expression analysis.
As anti-HIV vaccine candidates, attenuated poxvirus vectors have a number of desirable features, including promising safety profiles, the ability to incorporate substantial genetic material for the expression of foreign gene products and the capacity to efficiently activate the host immune responses to virally expressed antigens [16] [19].
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