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This concentration was necessary to ensure enough material for downstream analysis (PST determination and gene expression analysis).
All the samples yielded enough material for downstream analyses.
All samples yielded enough material for downstream analyses.
All the samples yielded enough material for downstream analyses and had a RIN equal or superior to 6.5.
A great challenge in secretomics is how to trigger a pathogen to secrete proteins as starting material for downstream analysis.
To overcome, a "pre-amplification" step is frequently performed where several genes are first coamplified by multiplex PCR to generate adequate material for downstream qPCR analysis of individual genes.
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Only if these controls revealed negative results, the material was used for downstream experiments.
As the arrays require different RNA preparation protocols and analysis to 'standard' Affymetrix arrays, the study serves to validate this novel use of the arrays themselves, the upstream protocols used to prepare material for hybridisation, and the downstream computational biology tools used for their analysis.
Mammalian cell culture material is often difficult to produce accurately and reproducibly for downstream studies.
After merging data sets (supplementary table S1, Supplementary Material online), a total of 291,096 overlapping autosomal SNPs remained for downstream analysis.
SNP variants detected among our genomes and used for downstream analysis are reported in supplementary data, Supplementary Material online, in a tab-delimited format.
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