The research on the rock bolt at home and abroad has been conducted mainly focused on the development of new rock bolt methods (shape, material, fixing method, etc).; however, the experimental study on the reinforcing effects of the rock bolt is insufficient.
Ten ml of BAL fluid was stored in RNALater (Ambion, Austin, TX, USA) at −70°C and cell blocks were made by pelleting the material, fixing in 10% formalin and embedding in paraffin.
ApoE KO-cuff mice (a, b) had flow-altering devices implanted around their carotids visible as transparent or reddish material fixed with a black suture (asterisks).
Allen is also fond of a clip featuring a 3D web of black material fixed to a real concrete wall, around and through which little arcs of light jump and disappear. .
To calculate the AP (see Supplementary Material), fix the retrieval algorithm A and consider a particular query q to a fixed database.
Similar results were achieved by Hamatani et al. [ 12], who found better PCR results after using lithium carbonate (Li2CO3) to discolor the paraffin-embedded material fixed with Bouin's solution prior to RNA extraction.
The histologic material fixed in 10% formalin and embedded in paraffin was cut into 4 μm slides and they were incubated for 12 h at 37°C, dewaxed in a histological clearing agent, Histo-Clear (National Diagnostics, Atlanta, GA, USA), and hydrated.
Provided that the concentration of vacancies is constant over time, such interdiffusion implies that the fluxes of the substitutional components in a binary solid solution take identical values and have opposite directions in the material fixed frame of reference.
Notably, archival material fixed with Bouin's solution showed a very strong background using the H3K27M antibody and poor H3K27me3 staining, and were therefore excluded from further analysis (n = 7, Supplementary Fig. 1a).
Resin-embedded material fixed in glutaraldehyde was sectioned at 2 µm and stained with periodic acid-Shiff's reagent (PAS) followed by 0·2 % toluidine blue in water (Feder and O'Brien, 1968) to observe insoluble carbohydrates and nuclei.
Some rare species for which we only had material fixed in formaldehyde could not be sequenced (Table 2) and we choose not to sequence Styela plicata because hundreds of sequences have been recently obtained for this species in the same study area (Pineda et al. 2011).
