The extraction conditions such as SBSE solid phase material, extraction time, extraction temperature, pH value and salt concentration were optimized.
Four independent variables such as extraction temperature (°C), ratio of water to raw material, extraction time (h), and number of extraction (n) were investigated.
The optimum conditions, using the Taguchi method, were obtained as: 1 14 (w/w) solid to water ratio, 25 mm size of the plant material, extraction time of 1.5 h and 500 W power.
The highest yield was obtained when extraction temperature, ratio of phosphate buffer to raw material, extraction time and ratio of trypsinase to raw material were 40 °C, 2, 5.5 h and 1.6, respectively.
The results showed that extraction temperature, ratio of phosphate buffer to raw material, extraction time, and ratio of trypsinase to raw material were the main four variables that influenced the yields of extracts.
Box Behnken design was applied to evaluate the effects of four independent variables (ratio of water to raw material, extraction time, extraction temperature, ultrasonic power) on the yield of PPP.
The optimum extraction conditions were as follows: extraction temperature 94 °C, ratio of water to raw material 9, extraction time 2.5 h, and number of extraction 5.
Results showed that the optimum extraction conditions were as follows: extraction temperature, 95 °C; ratio of water to raw material, 16; extraction time, 2.5 h; and extraction frequency, 4.
Three independent variables, including extraction temperature (°C), ratio of water to raw material, and extraction time (h), which significantly affected the yield of polysaccharides, were investigated.
Effects of various variables, such as applied power, ratio of solvent to material, and extraction time on the yield by two different technologies were investigated.
Four independent variables (ethanol concentration, pH value, ratio of liquid to material and extraction time) were investigated and the optimal conditions for BAAs were evaluated by means of response surface methodology (RS M.
