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The material eluted from Sepharose-bound anti-p15E antibodies was devoid of chemotactic and polarizing activity and suppressed the polarization and migration of monocytes in response to chemoattractants.
Material eluted from murine CD1d molecules that were purified from transfected insect cells included mainly PC and phosphatidylethanolamine (PE) [7].
Input material (whole cell extract, WCE), flow-through, wash, and material eluted from the beads were then analyzed by immunoblotting with an antibody directed against RPC1.
The majority of the material eluted from Q-Sepharose around 300 mM NaCl and contained rhamnose, glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine, as determined by sugar analysis.
A more quantitative measurement was performed on the material eluted from spot number 6 by quantitative amino acid analysis which indicated a total peptides concentration of 180 ng (data not shown).
Material eluted from beads was collected and shown in lanes labeled 'elution'elution
Similar(52)
Absorbance of material eluting from the column was detected at λ = 300 nm (UV-A).
The majority of breast tumour extracts contained mitogenic material eluting from the HPLC system at the TGF-alpha calibration point.
Finally, the bound material was eluted from the beads using 80°C 1× SDS loading buffer (Invitrogen) and resolved on 4 12% PAGE (NuPage, Invitrogen), followed by protein band detection with Coomassie Blue R (Sigma) staining.
The bound material was eluted from the beads by directly boiling in SDS sample buffer.
The bound material was eluted from the column using 0.1 M glycine, pH 2.3 and 0.5% Triton-X100.
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