Recovered plants had tested positive for FDp infection (also showing strong specific symptoms) at least one year before material collection for our proteomic analysis, and did not show symptoms or test positive for phytoplasmas in the following years.
We thank (late) Dr. Kris Morgenstern for making F1 crosses; Dr. Tim Boyle for making F2 controlled crosses; Debby Barsi, Moira Campbell, and Stephanie West for raising, cloning, and maintaining the mapping population and plant material collection; Joy Roy, Nicholas Buckley and Wendy Wiesser for their laboratory assistance in microsatellite genotyping.
Graphical abstract Two Islamic manuscripts from the historic reference material collection at UCL institute for sustainable heritage.
In RT patients, also the efficiency of different approaches for material collection was compared by employing superficial swabs from the tonsillar surface or the pharyngeal wall vs. fine needle aspirations or surgically prepared tonsillar core [12] [16], [21], [23], [24], [26].
We thank Jia-Yue Jiand, YangSheng Wang, Zhuo-Chen Wang, Yi-Luo Wu, Zhen Zhu, Lin-Li Lu and Yang Luo for material collection and RNA extraction.
All biomass was steam-pretreated in a steam gun unit consisting of a 19 litre reactor vessel and a cyclone tank for material collection.
Though feasible, such neoadjuvant studies require careful planning, enormous logistical support for material collection, and efficient screening systems to identify appropriate patients, all of which significantly increase the complexity and cost of conducting such research.
No specific permissions were required for plant material collection in the field studies.
Successful genetic epidemiological studies depend on the extraction of DNA of adequate quantity and quality, both of which are influenced by the method and tissue used for biological material collection [ 4].
The logic of material collection, i.e., the search for papers, and the paper validation test criteria, i.e., the decision to select a paper for the sample, are documented to ensure the replicability of the sampling process.
