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The Afrotropical region is undoubtedly under-collected and material collected is often insufficiently studied.
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The speech material collected was of approximately 40 min for DB1 and 140 min for DB2.
The material collected was reviewed to better understand the floor layers' viewpoints on sustainability.
The amount of material collected was determined gravimetrically.
Similarly, all the plant material collected was dried in shade, powdered in an electric grinder.
Any biological material collected was appropriately transported and stored until processing.
ex A. Rich., voucher specimen number 582251.0 Plant material collected was dried in an oven at 60 °C until dry.
For the uninduced master control, very little protein was eluted (<5 μg; Figure S1C, Lane 1) and all the material collected was processed for analysis.
At time of surgery, material collected was immediately fixed in a 10% buffered formalin, embedded in paraffin and stained with hematoxylin and eosin for light microscopy.
The materials collected were dewaxed by washing in xylene and then by rinsing in ethanol.
The German biologist Hugo Schausinsland spent three months on Laysan; material he collected was the subject of numerous articles.
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