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The period of 20 days is sufficient for composting for proper degradation and stabilization of waste material as previously been studied during in-vessel composting (Kalamdhad and Kazmi 2009).
Total RNA was extracted from 250 mg of plant material as previously described [ 42].
Total protein was extracted from 1 g of ground plant material as previously described [ 77].
The total genomic DNA was isolated from fresh and dried material as previously described using the modified CTAB method [ 34].
Immunohistochemistry was performed on formalin-fixed paraffin-embedded normal colonic mucosa, obtained with local ethics committee approval from archival material as previously described (Woolard et al, 2004).
Staining of endothelial cells by Factor-VIII A-00822, Dako, Copenhagen) was performed on formalin-fixed and paraffin-embedded archival material as previously published (Straume and Akslen, 2001).
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If the drop in the force curves can be attributed to the rearrangement of atoms to a new or modified crystalline structure, it appears that BCC materials undergo a significant change in their structure and subsequent realignment relative to the FCC materials, as previously reported in the literature.
Trans-capsular inside-out meniscal repair was performed using three different suture materials as previously described (Iuchi et al. 2017).
The Caco-2 cells were grown in 48 well plates and exposed to 95/1000 cells resuspended in DMEM, or other materials, as previously described.
These two oil peaks agree with the bi-exponential behavior of oil in oil-containing materials as previously suggested [ 25, 50].
In total, 39 formalin-fixed and paraffin-embedded (FFPE) NPC patient biopsy samples were selected for immunohistochemical analysis of EZH2 from an archival bank of materials as previously described (cohort 'A').
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com