In segmented-flow technology, sample material is suspended in aqueous segments of nano- or microliter volume and transported by a water-immiscible carrier liquid.
This original method is being replaced by a process in which the plant material is suspended on a grid above the water level, and steam from a second vessel is introduced under the grid.
100 mg material was suspended in 750 μl of CTAB buffer (2% CTAB; 100 mM Tris-HCl, pH 8 0; 20 mM EDTA; 2.5 M NaCl) and then incubated at 65°C for 2 h with occasional shaking.
The core material was suspended in an aqueous solution containing one of these enteric polymers, fed into the tubing and mixed continuously with a sodium sulfate solution as an antisolvent to induce coacervation.
The researchers at MIT also changed the microscopic structure of the material in a way that traps air near the surface, so that oil on the material is suspended partly on air, which prevents the oil droplets from sticking to the surface, Youngblood says.
The scraped material was suspended in sterile water containing 0.05% Tween-80 and stored at 4 °C.
The dry sample material was suspended in 1 ml of distilled water and then was centrifuged at 5000 × g for 5 min.
The material was suspended in deionized water and sonicated for 50 min; the homogenous suspension was deposited in the zetasizer cuvette and examined for particle size and zeta potential.
The algal/cyanobacterial slimes are anchored to rocks and not easily moved, but the Fe3+ (hydr oxide floc material is suspended in the water column, and could easily be washed downstream during winter storm events.
