To estimate the levels of endogenous MeJA, plant material was extracted by solid phase micro-extraction without the derivatization step.
When no cushion enrichment step was performed, 1 ml of packed material was extracted with 1 ml of polysome extraction buffer as described above.
25 g of ground plant material was extracted successively with distilled H2O in Soxhelt extraction apparatus.
The plant material was extracted using crude alcohol (70%) by maceration and Reflux extraction after optimization.
DNA from plant material was extracted according to the previously described protocol using chloroform-isoamyl alcohol extraction [ 36] with some modifications.
Genetic material was extracted and analyzed with a machine from 454 Life Sciences, a company immersed in the race to make gene sequencing a fast, cheap technology.
The amount varies depending on the amount of prepressing, the type of material being extracted, and the efficiency of the extracting system.
Sample preparation, in analytical chemistry, the processes in which a representative piece of material is extracted from a larger amount and readied for analysis.
The material was extracted by traction after 6 months.
Approximately 10 g of pulverized plant material was extracted with 100 mL solvent.
