Sentence examples for material and purified from inspiring English sources
Two sample types were selected for mRNAseq analysis, infected wheat leaf material and purified haustoria.
Comparison of starting material and purified material indicates the excellent recovery of the monomer, reasonable recovery of the dimer, and no recovery of the trimer.
Total RNA was extracted from both the infected wheat leaf material and purified haustoria using TRIzol reagent (Invitrogen, UK) and treated with DNA™-free DNase (Manufacturer) and the Removal Reagents kit (Ambion, UK) following the manufacturer's instructions.
Total RNA was extracted from ex planta material and purified haustoria using the RNeasy mini kit (Qiagen, Valencia, CA, USA) and the Absolutely RNA® miniprep kit (Stratagene, La Jolla, CA, USA), respectively.
The resulting DNA fragments were reamplified by PCR because of the low RNA copy number in the original material and purified by using the QIAquick PCR purification kit (Qiagen, Valencia, CA).
Monoclonal antibodies and recombinant DNA biopharmaceuticals are derived from relatively homogeneous source materials and purified by multistep schemes that are robust and amenable to scientific analysis and engineering improvement.
TK prepared the materials and purified the herbal extracts.
In brief, DNA was extracted using a standard phenol chlorophorm protocol, PCR was performed using the PCR primers and conditions provided in supplementary table S2, Supplementary Material online, and purified PCR products were directly sequenced on an ABI Prism 3130 Genetic Analyzer.
As a comparison, the classical ScMVD (sequence available in Fig. S3 in the supplemental material) was expressed and purified in the same way as put PtMVD and tested on the same substrates.
The catalytic powder, the composite material and the purified nanotubes have been analyzed by SEM, TEM and BET nitrogen adsorption.
Cross-linking of the starting material and partially purified samples followed by Western blotting showed that tetramers and related multimers of αSyn are abundant in post-mortem tissue and are readily detectible at intermediate stages of purification; however, the final chromatography step which purifies αSyn to homogeneity can destabilize the native multimers.
