Soluble scFvs were produced in HB2151 bacterial strain, as described in Materials and Methods, and normalized by ELISA on anti-polyhistidine antibody-coated plates.
Our approach of using mixed-stage starting material and combining normalized/non-normalized cDNA pools was successful in producing a transcriptome with high gene representation.
Based on a study of 25 different types of materials, the normalized undrained shear strength of clay shales was found to correlate satisfactorily with overconsolidation ratio following the SHANSEP (Stress History and Normalized Soil Engineering Properties) normalization procedure for clays.
CD59 transcripts in an adult gecko spinal cord were detected by preparing four segments along the anterior-posterior (AP) axis (i.e. cervico, thoracic, lumbar and sacral segment, refer to Material and Methods), and were normalized to endogenous EF-1α.
We determined the change in GFP fluorescence intensity either in the colocalization mask generated with our algorithm or in a manually generated mask (see Materials and Methods) [20], [22] and normalized to GFP intensity in the whole cell.
All enrichments are calculated over input and normalized (see 'Materials and methods').
Results for each polymorphism were expressed as ratio between the two alleles and normalized to the starting material which was set to 1 to reflect the condition of a heterozygous genomic DNA.
Particle size distributions obtained pre- and post-exposure to high and low shear of each material batch were normalized and compared.
The 44 arrays derived from these animals were preprocessed and normalized as described in the Materials and Methods and differential expression was assessed between the two groups.
Model diagnostics are provided in Supplementary Materials and Methods online (Supplementary Figures S1 – S3 online) and normalized visual predictive checks are shown in Supplementary Figure S4 online.
