Sentence examples for material and incubated from inspiring English sources

MH broth was inoculated with bacterial colony material and incubated overnight at 37°C.

Nucleosomes were assembled to yield greater than 90% nucleosomal material and incubated with hSWI/SNF.

200 pmol of biotinylated DNA oligos (Invitrogen) were added to 1 mg of material and incubated at 4°C for 4 h.

For RNAse sensitivity, Superasin was omitted and 10 μg of RNAse A (Ambion) was added to sonicated material and incubated at 37°C for 30 min before biotinylated probe was added.

Proteinase K (100 μg) and Glycogen (20 μg) were added to the eluted ChIP material and incubated for 2h at 37°C, and DNA was twice extracted with phenol-chloroform-alcohol isoamylic acid, and precipitated with ethanol/NaCl.

Lysis buffer (800 µl) was added, samples were incubated for 20 min at room temperature, and insoluble material was removed by centrifugation at 14,000× g for 5 min. Anti-FLAG M2 beads (50 µl of a 50% slurry) were added to the soluble material and incubated for 1 hr at 4°C.

Initially, 1 × 104 cells were seeded into each well containing 200 μL cell culture mediums in 96-well plate and incubated for 24 h and then added the relevant materials and incubated at 37°C with 5% CO2 for 72 h.

Antibodies were diluted according to supplementary material Table S2 and incubated overnight on a rotator at 4°C.

Capture disks with isolated material were microcentrifuged and incubated in the extraction buffer for 30 min at 42ºC.

For each scute 0.25 0.5 g material was milled and incubated overnight in 2 ml EDTA buffer, 200 μl N-Laurylsarcosidase and 20 μl Proteinase-K followed by a phenol-chlorophorm extraction with a final concentration step using Centricon©-100 columns.

All strains were plated out onto appropriate medium (for details see "Materials and methods") and incubated.