Libraries for the 12 genes were prepared and individually barcoded for high-throughput pair-end sequencing using MiSeq2500 (Additional file 5: Material and Methods; Additional file 9: Table S5).
We can host supplementary figures and legends, detailed materials and methods, video files, audio files, original data files, and large data sets.
We can host supplementary figures and legends, detailed materials and methods, video files, audio files, original data files, source code, and large data sets.
The film was seized as obscene material, and its distributor filed a court case and an appeal in 1974, but lost both.
Following removal of contaminants (see Material and Methods, Additional file 1C, D) reads from both libraries were pooled and assembled using the Newbler Assembler from Roche.
By the time he graduated, Chris was working closely with a start-up company to commercialize his materials and had filed five patents for his inventions.
These were easily identified by deep genomic sequencing of the parental and mutated chromosomes (Materials and Methods; File S1).
In addition to our own chlorine measurements, water quality reports have been included as supplemental material (File S1 and File S2).
Examples of RelocaTE searches using TEs where only the TSD length is known can be found in the supporting material (Table S4 and File S1).
Separate scripts for integration of R and bioconductor data formats with these programs were developed and are included as supplementary material (Additional files 3 and 4).
