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CD1 females mated with vasectomized CD1 males were used as pseudopregnant mice.
Females mated with vasectomized males generated 42% more stem cells than virgin females did.
The next morning morulae and blastocysts were transferred into the uteri of 2.5-days post-coitum (dpc) pseudopregnant CD-1 (Charles River) females previously mated with vasectomized males.
Recovered embryos were transferred into the oviducts of pseudopregnant females at 0.5 dpc that had been mated with vasectomized ICR male mice.
The following day, embryos, which had developed into blastocysts, were transferred into the uterus of pseudopregnant recipient CD-1 females mated with vasectomized CD-1 males 2.5 days earlier.
When cloned embryos were developed to the 2-cell stage, it was transferred to the oviduct of foster mothers (ICR strain) which had been mated with vasectomized ICR males 1 day previously.
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These had been mated with a vasectomized ICR male the night before transfer.
8-10 week old CD-1 (Charles River, Wilmington, MA) female mice were mated with fertile or vasectomized males the same strain (10 16 weeks) to induce pregnancy or pseudopregnancy, respectively [18].
To induce pregnancy or pseudopregnancy, adult female mice were mated with fertile or vasectomized males of the same strain (day 1=day of vaginal plug).
Two lions have mated with their daughters.
I mated with all the women.
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