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The resulting strains (containing 0 3 copies of either mgs or gldA) were mated to create all possible combinations of the 2 genes introduced for 1,2-PD production.
Animals from the F2 pool were randomly chosen and mated to create a F3 population in this manner.
Haploid a and α strains were isolated by tetrad dissection and subsequently mated to create diploids homozygous for the resistance.
Fifty different combinations of pathway and cDNA libraries were mated to create diploid screening libraries.
The Tg F1 generation were mated to create homozygous Tg RE:HSE EGFP) line.
The F1s were randomly mated to create 200 C1 S0 plants.
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Using a forward simulator (see File S1), we simulated a 50-50 adminture in a single generation followed by six generations of random mating to create a simulated population of 800 diploid individuals.
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