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After collecting the eggs, the scientists mated the same females with red-banded males.
Within populations, all females mated the same number of times.
In addition to the random genome shuffling strategy described above, we mated the same eight parental strains using a targeted funnel-breeding scheme based on non-stable plasmid-based dominant markers (see Figure 2A and ' Methods').
We then estimated the relatedness of the queens to their male mates and the relatedness of males mated to single queens to determine if inbreeding occurred or if related males mated the same queen.
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The third cross (WxM) was obtained by mating the same W boar with four Meishan (M) sows.
Allocation of mates (sires) to queens is random, but each queen within a full-sib group is mated to the same sire and each sire is mated to the same number of queens.
Mutants and controls were mated at the same time.
Daughters that inherited nests mated with the same number of males as their mother.
These mice were mated in the same manner of control group.
We then turned our attention to the reproductive skew of males mated to the same queen.
To obtain large full-sib families, each F1 sow was usually mated to the same sire during the different parities.
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