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Traits implicated in this syndrome include large colonies, colonies with multiple queens that mate within the nest, and colonies that often occupy many interconnected nests with little to no territorial aggression among them [25].
Wingless males stay lifelong in their natal nests, but the behaviour of winged males is more flexible: they mate within the nest early during their adult lives, but later disperse and search for female sexuals outside [7], [12], [13].
Adults will mate within the first day after eclosion if adequate food is available (Milani 1975).
Males that did not mate within the 2 hr window were scored as being unsuccessful.
Firstly, 88% of the D. subobscura that mate within two hours mate within the first 60 minutes.
Males did not attempt to mate within the first 5 min with young hermaphrodites that still possessed self-produced sperm.
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It most likely began with the evolution of facultative mating within the nest, readoption of some newly-mated queens, and nest budding, leading to the extended family colonies that are typical for extant L. turcicus in lowland populations.
Adults from each treatment group were caged together and mated within the group.
The probability of mating within the 30 minutes of observation (i.e. the latency to mate) was significantly influenced by Relatedness.
In fact, using the present protocol in earlier experiments, we have reliably observed nearly 100% of females mating within the first hour of being combined with males.
At some localities in France where the Z and E races of O. nubilalis co-occur, strong assortative mating within the races was found to be occurring [22] [24].
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