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The study showed that if wild females preferred to mate with genetically engineered males and if those matings produced offspring that did not survive well, wild populations could be wiped out, a result they called the Trojan gene effect.
Alternatively, the disassortative mating hypothesis [3] states that females should prefer to mate with genetically dissimilar males in order to produce offspring with high levels of heterozygosity.
Contrary to our expectation, we found that offspring were less genetically diverse (multi-locus heterozygosity) than expected under a random mating, suggesting that females tended to mate with genetically similar males.
Our prediction that females would tend to mate with genetically dissimilar mates was based on the assumption that this insular population has low genetic diversity and that it is therefore vulnerable to inbreeding depression.
Overall we found that 29% of litters had multiple sires, but we found no evidence that females were more likely to produce multiple-sired litters when they had the opportunity to mate with genetically dissimilar males compared with controls, regardless of whether females were inbred or outbred.
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Mating with genetically dissimilar individuals is thought to be beneficial because increased genetic diversity reduces the risk of inbreeding depression, and ensures a range of genotypes for offspring to contend with environmental uncertainty [ 2, 24].
Material benefits are unlikely in the stickleback system, but polyandry may lower the probability of mating with genetically incompatible, inferior or infertile mates as well as increasing next-generation genetic diversity and mean offspring fitness [ 21, 22].
If genetic differentiation between populations develops, gene flow between populations can introduce traits or alleles which are disadvantageous in the local population and this may lead to organisms within these populations evolving mechanisms that prevent mating with genetically distant populations, eventually resulting in the appearance of new species.
One such explanation is avoidance of costs associated with mating with genetically incompatible males.
In V. canescens, females use volatile compounds to avoid mating with genetically related individuals [ 40].
Both statistics were significantly higher in clonal couples, indicating a trend for outbreeding in these native populations (i.e. mating with genetically distant individuals).
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