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In this process haploid M and P cells first mate to form diploid zygotes, which then enter meiosis and sporulate.
At high population density two amoebae of compatible mating types may mate to form a mononucleate diploid zygote [ 13].
Second, the population of haploid meiotic cells consists of equal numbers of the two mating types, which when plated could mate to form diploids.
S. pombe (Sp) and S. kambucha (Sk) generally exist as single-celled haploids, but cells of opposite mating types readily mate to form single-celled diploids.
In this process haploid P and M cells first mate to form dipoid zygotes [ 4], which then undergo meiosis and sporulation [ 5].
Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha, which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes.
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Because pycniospores are haploid cells in which mating type has segregated and are primed for mating to form the dikaryotic aeciospores, they are expected to express mating-type specific genes.
When hyenas want to coordinate clan mates to form coalitions against lions, they produce a vocalization, called "lowing," that sounds very much like the mooing of a cow.
S. cerevisiae strain S288c (204508; ATCC) (American Type Culture Collection, Manassas, VA) was used as the haploid, and then mated to form the homozygous diploid for the diploid experiments.
The Saccharomyces cerevisiae strains S288C and RM11 were grown overnight at 30° on an agar plate with the YPD rich medium, and mixed on an YPD plate to allow mating to form diploid cells.
Subsequent generations were generated by selecting 300 males and 300 females among the selection candidates on their estimated breeding values and performing random mating to form 300 full-sib families in each generation, assuming non-overlapping generations.
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