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Primers matching to repeat databases (Jurka et al. 2005) were excluded.
Where not otherwise stated, BLAST [ 39] programs were used with an e value cutoff of 1E-6, and repetitive sequence filtering on except when matching to repeat databases, where the filter was off.
After removing the small RNAs matching to repeat, rRNA and tRNA, the remaining 25,802,718 small RNAs were subjected to miRNA identification.
Similar(57)
Consistent with these generalizations, several of the tarantula ESTs showed significant homology to paramyosin[ 12], and matches to repeated, sequential Ig and Fn motifs characteristic of titin-like proteins were also found by InterProScan.
Similarly, our data revealed that distinct sRNAs reads matching to repeats in in planta growth were ~10% higher than in the mycelial libraries (Additional file 5: Figure S5A and B).
However, significantly more sRNAs in T. pseudonana (561,235 sequences, 1.9% of mapped sequences) matched to repeats than in F. cylindrus (146,818 sequences, 1.6% of mapped sequences) (Additional file 3).
Djokovic, who played a solid match to repeat as champion, was the tougher player physically and mentally as he won his 30th tour title and 11th A.T.P. Tour Masters final.
Our method evaluates the support for new sequence junctions present in a clonal sample from split-read alignments to a reference genome, including matches to repeat sequences.
This reads in GFF files of various types of protein or transcript genomic alignments and of other types of genomic features such as matches to repeat sequence libraries.
BESs regions matching NCBI NR proteins and not matching to RepBase repeats [ 57] were assumed to contain protein-encoding genes.
Therefore, reads matching to unmasked repeats, which are more than 10% divergent from the consensus sequence, in the div10_low method widely determined the div10_low higher read depth.
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