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In category I, the miRNA-guided cleavage fragment was the most abundant tag matching the transcript; therefore, category I is most reliable for the detection of miRNA-targeted genes.
Only small RNAs matching the transcript coding part of the nuclear genome (excluding all non-protein coding structural RNAs and organellar small RNAs) were used as input and matched against the Arabidopsis genome.
Probe annotation: depending on the target organism, updated genome annotation can considerably affect differential expression estimates for 30 40% of all the targets of an Affymetrix chip (Dai et al., 2005) We therefore used the 584 345 probes of the HuGene-1.0-ST-v1 chip matching the transcript annotation of EnsEMBL r58 (custom CDF v13).
In category I, the miRNA-guided cleavage fragment was the most abundant tag matching the transcript; In category II, the miRNA-guided cleavage fragment was not the most abundant fragment; however, it still formed a clear peak in the T-plot.
Nine target ESTs were classified as category 0. Category 0 targets are transcripts where the degradome reads corresponding to the expected miRNA-mediated cleavage site were the most abundant reads matching the transcript and there is only one peak on the transcript with more than one raw read at the position.
Similar(55)
The sRNAscanner-predicted sRNA6 overlapped with a previously proposed processed 5'UTR fragment of the yhiI transcript [38] that was likely to match the transcript we detected under ESP-2.0 ESP-2.0ons.
The 21 dpi cyclops roots were compared to wild-type 1, 3, 7 dpi roots aiming to identify a particular stage of symbiosis which matched the transcript profile of cyclops at 21 dpi (Folder S4).
In brief, gene symbol annotated, A grade probe sets (probe sets for which the manufacturer confirms most probes match the transcript perfectly) with sufficient signal intensity (>4 chips with ≥4.2 signal intensity) for reliable measurement were retained.
But the most damning words match the transcripts and the audio, and do not seem out of context.
Clark Hoyt, a former public editor for The New York Times, reviewed O'Keefe's raw footage and edited tapes and concluded that "the most damning words match the transcripts and the audio, and do not seem out of context".
The first author transcribed and translated all interviews into English and matched the transcripts with the notes and memos she had taken during the data collection process [ 31].
More suggestions(15)
matching the transcriptional
matching the governor
matching the inside
matching the money
matching the interest
matching the hype
matching the gain
matching the C-Factor
matching the query
matching the strength
matching the raffle
matching the turbulence
matching the shambling
matching the texture
matching the recognition
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