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Exact(11)
For the June 2007 sample, matching reads were distributed as follow: 69.9% (viral origin), 23.8% (bacterial origin), 0.05% (archaeal origin) and 6.1% (eukaryotic origin).
The unique matching reads were used for further analysis.
The matching reads were clustered and those that had more than five reads were analyzed further.
Sequence reads were mapped to the mouse reference genome (mm9) using Bowtie, and all uniquely matching reads were retained.
Where tags could be assigned to up to 10 multiple paralogs, matching reads were split equally between them.
Read counts for these transcripts were analyzed, and any sequence where all of its matching reads were unique alignments was considered valid (Table 3).
Similar(49)
Where multiple precursor loci predicted identical mature miRNAs, only the precursor with the largest number of matching reads was reported.
If only exactly matching reads are admissible, the average number of aligned reads per position drops to 846.95 (median 587).
Matching reads are trimmed to remove any portion that aligned to the TE, and a note of which end of the read was trimmed of TE sequence is appended to the sequence name (start or end).
A similar percentage in matching reads was also obtained when sequences were trimmed for low-quality bases before mapping or when the whole genomic sequence of C. intestinalis A was used as a reference (data not shown).
Second, when the number of Sorghum gene models with a minimum of five matching reads are compared among libraries from similar tissues, a substantial number of gene models appear to be uniquely represented in only one library.
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