Sentence examples for matching normal sequences from inspiring English sources

Sequence changes (e.g. indels, base substitutions) were identified by finding base changes between each tumor and its matching normal sequences.

Mutations were identified using an automated mutation caller and then verified manually with comparison made to the matched normal sequence in the case of all primary tumor samples.

On a human melanoma, sequenced to 60× coverage with matched normal sequenced to 30×, Socrates predicted about 105 000 SVs in the tumour when run using its most sensitive settings (in comparison Delly predicted ∼150 000; BreakDancer 7800).

No matching normal tissue is sequenced because these mutations are typically only present in cancer cell genomes, and because the assays are quite focused in their scope, detecting mutations only in restricted, commonly mutated regions.

In order to identify mutations, if any, in the SKCG-1 allele that is still present in the tumour, genomic sequence of SKCG-1 (from base +1 to +1692) was amplified from DNA of tumour as well as from matching normal tissue and sequenced.

We sequenced tumor and matching normal colon tissues from two patients with high grade adenocarcinoma of the colon (G3), patient 1 with a microsatellite instable and patient 2 with a microsatellite stable tumor (Table 1, Figure S1).

We performed high-throughput cDNA sequencing in colorectal adenocarcinoma and matching normal colorectal epithelium.

We isolated a differentially expressed sequence (F1. 4) in a DD analysis of RNA from HNSCC and matching normal tissue.

Cancer tissue contained only one sequence-altered allele of the gene ATP50, which was present heterozygously alongside the wild-type allele in matching normal epithelium.

No matched normal exome sequencing data are required to run this pipeline.

The prevalence of D310 mutations increased significantly with the number of cytosines in the matched normal tissue sequence (P=0.02).